1. pMSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro lentivector plasmid
Quantity: 10 µg 2. pMSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro piggyBac transposon plasmid
Quantity: 10 µg 3. MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro Stable Jurkat cell line (Puro resistant)
Quantity: 5 x 10^6 Cells/mL
Advantage: Choose a mouse Foxp3 overexpression system with your choice of lentivector- or PiggyBac-based transgenesis, or Foxp3-overexpressing Jurkat cells